WebJul 15, 2002 · Before all experiments described below, cells were incubated for 24 hours in serum-free Ultraculture (BioWhittaker) medium. Thereafter the cells were centrifuged and, for complement treatment, resuspended in fresh Ultraculture medium and seeded into 24-well plates at a density of 1 × 10 6 cells/mL. Complement components at concentrations ... WebMay 22, 2006 · After 5 days, the medium was changed and a serum-free medium (UltraCulture, BioWhittaker Europe, Verviers, Belgium) was used for further propagation. The cell cultures were investigated by phase contrast microscopy. Specimens that showed intracellularly growing microorganisms underwent further characterization.
BioWhittaker® UltraCULTURE - ys-bio.cn
WebBioWhittaker® UltraCULTURE™ Serum-free Medium Turbidity may develop UltraCULTURE™ Medium. Experiments have determined that the turbidity will not alter … Web(Clontech). The medium was replaced after 12 hours with BioWhittaker Ultraculture medium (Lonza) and 48 hours post-transfection the viral supernatants were harvested, filtered, and concentrated by using Centricon Plus-70 Ultracel PL-100 (Millipore). Murine 3T3-L1 preadipocytes (American Tissue Culture Collection, Manassas, VA) were datax mysqlwriter update
Ultraculture With Jason Louv on Apple Podcasts
WebUsing different strategies, three mouse hybridoma lines (IgGl, IgG2a, IgM), were adapted to grow in three different serum-free media (BioWHITTAKER): UltraCULTURE TM, U1traDOMA TM and UltraDOMA-PF TM.According to the characteristics of clones, adaptation to UltraDOMA TM and UltraDOMA-PF TM started from UltraCULTURE TM … WebMay 25, 2000 · Thus, serum-free media such as UltraCULTURE allow multiple collections for extended periods of time. This is an attractive feature for virus production at a preparative scale. Web– The formulation for UltraCULTURE™ Media has been submitted to the FDA as a product Master File PC-1™ Chemically Defined, Serum-free Medium – Superior media for … data xlrd.open_workbook file_path