Web520 nm) Fluorescein isothiocyanate (FITC) is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly WebFor flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10 6 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. This FITC conjugated rat anti-mouse IgG antibody is useful for immunofluorescent staining with flow cytometric analysis. Park S, et al. 2015.
Mouse IgG1 kappa Isotype Control, FITC (11-4714-81)
WebBlue Laser (488 nm) Application Notes. Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes 3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a + cells. Webin FITC, PE, PerCP-Cy5.5, and APC, and you want to add a fifth color. PE-Cy7 is an obvious choice, since it is the brightest fluorochrome not already in your panel. ... BD FACSCanto™ flow cytometer), and upon reagents that can be generally purchased as catalog antibody conjugates. how did beowulf become king of the geats
Fluorochrome/Laser Reference Poster - University of Iowa
WebGeneral description. Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to ... WebFluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). WebBD Pharmingen™ Technical Data Sheet FITC Mouse Anti-Rat CD134 Product Information Material Number: 554848 Alternate Name: OX-40 Antigen Size: 0.5 mg Concentration: 0.5 mg/ml ... 559532 FITC Mouse IgG2b, κ Isotype Control 0.25 mg MPC-11 Product Notices 1. Since applications vary, each investigator should titrate the reagent to obtain optimal ... how did benny severide die on chicago fire